Overview
We profile who is present in a microbial community, what they can do and how communities differ across samples or treatments.
Salient features
- ASV and taxonomic profiling
- Assembly, binning and genome-resolved metagenomics
- Resistance gene screening and diversity statistics
Analyses we offer
16S rRNA-seq analysis
Profiles bacterial communities from 16S amplicons into taxa, diversity and differential abundance.
Methods: rachis, dada2, mothur.
Metagenomic sequencing analysis
Classifies and assembles shotgun metagenomes to reveal community composition and function.
Methods: kraken2, MetaPhlAn, megahit.
Amplicon sequencing analysis
Processes marker-gene amplicons (16S, ITS, 18S) into denoised sequence variants.
Methods: dada2, vsearch, rachis.
Metagenome binning & taxonomy
Recovers genomes from metagenomes and assigns standardised taxonomy.
Methods: metaWRAP, GTDBTk.
Antimicrobial resistance gene detection
Screens genomes and metagenomes for known resistance genes and mutations.
Methods: rgi, amr, abricate.
Microbial community statistics
Tests community differences with ordination, PERMANOVA and diversity metrics.
Methods: phyloseq, vegan.
What you receive
- A written report with methods, parameters and interpretation
- Processed data tables and publication-quality figures
- Scripts and settings so every result can be reproduced
Not sure which analysis fits your data? Contact us and we will advise.